安徽地方猪TLR4基因第3外显子的SNP分析

doi:10.11843/j.issn.0366-6964.2014.11.005

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1767-1774.doi: 10.11843/j.issn.0366-6964.2014.11.005

安徽地方猪TLR4基因第3外显子的SNP分析

丁月云，朱卫华，薛玮纬，孟云，黄龙，张晓东，殷宗俊^*

(安徽农业大学动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室，合肥 230036)

收稿日期:2014-03-27 出版日期:2014-11-23 发布日期:2014-11-23
通讯作者: 殷宗俊，主要从事猪遗传育种研究，E-mail：yinzongjun@ahau.edu.cn
作者简介:丁月云(1981-)，女，安徽蒙城人，博士，副教授，主要从事猪遗传育种研究，E-mail： dingyueyun@ahau.edu.cn
基金资助:
国家自然科学基金项目（31171200）；安徽省科技攻关重点项目 (1301031032)；国家农业科技成果转化资金项目（2012GB2C300207）；科技富民强县专项行动计划；安徽畜禽产业共性技术研究院资助

Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds

DING Yue-yun，ZHU Wei-hua，XUE Wei-wei，MENG Yun，HUANG Long，ZHANG Xiao-dong，YIN Zong-jun^*

(Anhui Provincial Key Laboratory of Local Livestock and Poultry Genetic Resources Conservation and Bio-Breeding，School of Animal Science and Technology，Anhui Agricultural University，Hefei 230036，China)

Received:2014-03-27 Online:2014-11-23 Published:2014-11-23

摘要/Abstract

摘要：

本试验采用 PCR-SSCP 方法对皖南黑猪、圩猪、安庆六白猪、霍寿黑猪及长白猪5个群体共354个样本TLR4基因外显子3部分片段的遗传变异进行了检测，旨在系统分析安徽地方品种猪TLR4基因的多态性，为探讨该基因在免疫和防御系统中发挥的作用提供依据。结果检测到2个突变：G417A、C1027A，其中，G417A为非错义突变，仅在皖南黑猪、圩猪和安庆六白猪群体中检测到，且只检测到GG、GA 两种基因型，在该3群体均为低度多态，且均处于哈迪-温伯格平衡状态(P>0.05)；C1027A为错义突变，且引起了编码氨基酸性质的改变，在皖南黑猪、圩猪、安庆六白猪、霍寿黑猪及长白猪群体中均检测到CC、CA、AA 3种基因型，在该5群体均为中度多态，且均处于哈迪-温伯格平衡状态(P>0.05)；χ²独立性检验结果表明，G417A、C1027A各基因型的分布在皖南黑猪、圩猪、安庆六白猪、霍寿黑猪及长白猪5个中外猪种间存在极显著差异(P<0.01)。结果提示，安徽地方猪TLR4基因外显子3区序列相对保守、多态含量较低，群体遗传变异程度较小；C1027A位点多态性在猪种间分布的差异是否与品种抗病性有关，值得进一步研究。

Abstract:

In this study，using the PCR-SSCP method，the genetic variations in exon 3 of TLR4 were detected among 5 pig populations including Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the total individual number was 354.This study was conducted to analyze the polymorphisms of exon 3 of TLR4 in Anhui native pig breeds and aimed to provide a theoretical foundation for further research on the role of TLR4 in immune and defense system.Two SNPs were found，including G417A and C1027A.G417A was a synonymous SNP site，2 genotypes (GG，GA) were detected only in Wannan Black，Wei and Anqing Liu White pigs，the 3 populations were all in low polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).C1027A was a nonsynonymous SNP site and changed the character of amino acid，3 genotypes (CC,CA，AA) were detected in Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the 5 populations were all in average polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).The χ² test results of independence for the genotypes distribution of each SNP locus showed that the genotype frequencies for Landrace and Wannan Black，Wei，Anqing Liu White，Huoshou Black pigs were significantly different (P<0.01) at G417A and C1027A loci.These results indicated that the exon 3 sequences of TLR4 were relatively conservative in Anhui native pig breeds，and these populations had low polymorphism and genetic variation.In addition，the association between the differential genotypes distribution of C1027A locus in multiple pigs and the different disease resistance capability for pig breeds was worthy of further research.

中图分类号:

S828
S813.3

丁月云，朱卫华，薛玮纬，孟云，黄龙，张晓东，殷宗俊. 安徽地方猪TLR4基因第3外显子的SNP分析[J]. 畜牧兽医学报, 2014, 45(11): 1767-1774.

DING Yue-yun，ZHU Wei-hua，XUE Wei-wei，MENG Yun，HUANG Long，ZHANG Xiao-dong，YIN Zong-jun. Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1767-1774.

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安徽地方猪TLR4基因第3外显子的SNP分析

Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds

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